Important publication on high-speed video-microscopy analysis
The laboratory of Bestcilia coordinator, Heymut Omran (University of Münster, Germany), has issued a vital publication on high-speed video-microscopy analysis. The publication describes the influence of different gene mutations on the ciliary beating patterns. By structurizing the knowledge on HVMA diversity, the article is likely to serve as a guide for PCD diagnostics, not only within Bestcilia project WP 3, but also for all doctors diagnosing PCD.
High-speed video-microscopy analysis (HVMA) of ciliary beating is presently the most frequently used first-line tool for PCD diagnostics. Although relatively cheap, HVMA requires specific knowledge and extensive experience. In the recent years, the spectrum of HVMA findings has been widely expanded from grossly abnormal to very subtle, often making the proper PCD diagnosis using HVMA challenging. The publication of Omran’s group in the recent issue of European Journal of Respiration is an important milestone in PCD diagnosis using HVMA method.
By using the overwhelming number of over 1000 HVMA videos from 66 patients with genetically confirmed PCD, Omran’s team has correlated the pattern of ciliary beating with the specific genotype of the patient. They show that variations in the ciliary beat pattern can indicate, which group of genes has been mutated in the patient. On the other hand, they also demonstrated that information on frequency of ciliary beating is not very helpful for PCD diagnosis.
The publication adds to the state-of-the-art knowledge of PCD diagnostics developed within Bestcilia program. By structurizing the knowledge on HVMA diversity, the article will not only serve as a guide for PCD diagnostics within Bestcilia project (WP3), but is also likely to serve as a guide for all doctors diagnosing PCD.
Full article can be accessed here